Protein_Domain

Part:BBa_K4803002:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


IFNGR1 extracellular domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization to ensure effective expression of this part in human cells. We also used simulations to predict and confirm that the steric structure is not disrupted even when the amino acid sequence is only in the extracellular domain.

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

This part is codon-optimized for sequences outside the plasma membrane from the IFNGR1 cDNA [1] listed in GenBank.

Reference

[1] https://www.ncbi.nlm.nih.gov/nuccore/NM_000416.3?report=genbank